SINGLE-USE BIOREACTOR
Одноразовий біореактор є інноваційним рішенням у біовиробництві, пропонуючи одноразову ефективну платформу для культивування клітин. Створений для гнучкості та зручності, він усуває потребу в очищенні та стерилізації, спрощуючи робочі процеси. Ідеально підходить для різноманітних застосувань у біовиробництві, він є економічно ефективним і масштабованим варіантом, який легко підтримує виробництво біологічних продуктів. View All Equipment.
НАЛАШТУВАННЯ
The setup of a single-use bioreactor involves several steps and components. Here is a general overview of the setup process:
Location and Environment: Choose a location with adequate space and proper environmental conditions. Ensure proper ventilation and access to utilities (power, water, and gas).
Inspect Equipment: Carefully inspect the single-use bioreactor components for any damage during shipping. Verify that all components are included as per the equipment list.
Prepare Work Area: Clean the work area to maintain a sterile environment. Use proper personal protective equipment (PPE) to prevent contamination.
Assemble the Bioreactor System: Follow the manufacturer’s instructions to assemble the single-use bioreactor system. Connect tubing, sensors, and other components according to the provided diagrams.
Prepare the Bioreactor Bag: Ensure the integrity of the single-use bioreactor bag. Connect the bag to the bioreactor system, making sure all connections are secure. Perform any required leak tests.
Sterilization: Sterilize the entire single-use bioreactor system using the recommended method (e.g., gamma irradiation). Follow the manufacturer’s guidelines for sterilization time and conditions.
Media and Buffer Preparation: Prepare the culture media and buffer solutions according to the established protocols. Sterilize the media using appropriate methods.
Sensor Calibration: Calibrate sensors such as pH, dissolved oxygen, and temperature according to the manufacturer’s specifications. Ensure proper functioning of all sensors.
Inoculation and Seeding: Inoculate the bioreactor with the desired cells or microorganisms. Follow the recommended seeding density and inoculation procedure.
Set Operating Parameters: Input the desired process parameters such as temperature, pH setpoints, agitation speed, and aeration rate. Set up the control system based on the specific requirements of your process.
Monitor and Control: Monitor the bioreactor during the cultivation process. Make necessary adjustments to control parameters based on sensor readings.
Harvesting:Follow the appropriate procedures for harvesting the cultured cells or microorganisms.
Disposal: Dispose of the single-use bioreactor bag and any other disposable components according to local regulations.
Documentation: Maintain detailed records of the entire process, including setup, sterilization, inoculation, and harvesting.
Post-Use Cleaning and Disassembly: If applicable, follow the manufacturer’s guidelines for post-use cleaning and disassembly of the bioreactor system.
Please note that this is a general outline, and the specific steps and procedures for setting up a single-use bioreactor may vary depending on the manufacturer, model, and application. Always consult the manufacturer’s guidelines and any applicable safety procedures before attempting to set up and operate it
КОДИ ПОМИЛОК
Single-Use Bioreactors can experience various error conditions during operation, which may affect the efficiency and quality of the biological process. Some common error conditions include:
Sensor Malfunction: Erroneous readings from pH, dissolved oxygen, or temperature sensors. Sensor drift or calibration issues.
Leakage in Bioreactor Bag: Detection of fluid leakage from the single-use bioreactor bag. Failure in the integrity of the bag material.
Contamination Events: Presence of foreign particles or microbial contamination in the culture. Unplanned introduction of contaminants during setup or operation.
Agitation and Aeration Issues: Inconsistent or insufficient agitation in the bioreactor. Aeration problems leading to inadequate oxygen supply.
Power Supply Interruption: Unexpected power outage affecting the bioreactor operation. Failure of backup power systems.
Temperature Fluctuations: Deviations from the set temperature range. Inability to maintain the desired temperature.
Pump Failures: Malfunction of pumps responsible for media or buffer circulation. Irregular flow rates affecting the process.
Inoculation Failure: Poor cell attachment or growth after inoculation. Inadequate seeding density.
Failure in Sterilization: Incomplete sterilization leading to microbial contamination. Issues with the sterilization process or equipment.
Data Recording Errors: Incorrect recording or logging of process parameters. Failure in data storage systems.
Pressure Buildup: Excessive pressure within the bioreactor system. Risks of bursting or damage to the bag.
Clogging in Tubing: Blockages or clogs in the tubing affecting fluid flow. Impaired nutrient delivery to the culture.
Sudden pH Shifts: Rapid and unexpected changes in pH levels. Difficulties in maintaining pH setpoints.
Failure in Harvesting Mechanism: Issues with the harvest valve or mechanism. Inability to collect the cultured cells efficiently.
Communication Failures: Loss of communication between sensors and the control system. Issues with software or hardware communication interfaces.
To minimize the occurrence of error conditions, it is essential to follow proper operating procedures, implement preventive maintenance schedules, and closely monitor the bioprocess parameters throughout the operation.